Synergistic Actions of Pyridostigmine Bromide and Insecticides on Muscle and Vascular Nociceptors

Abstract

We examined whether chronic exposure to combinations of two neurotoxicants (chlorpyrifos and permethrin) with pyridostigmine bromide (PB) could produce a delayed neuropathic pain condition in rats; and whether corresponding molecular changes would occur in nociceptive neurons coding for pain in skin, muscle or vasculature. Following a 30 day exposure to neurotoxicants/PB, we observed molecular dysfunctions in membrane Kv proteins (Kv7, KDR) that persisted at least 4 weeks after exposure had ended. K+ channel protein malfunctions were limited to neurons we have attributed to the vascular system. Neurons that innervate skin and muscle were not affected. The physical location of vascular nociceptive neurons renders them most exposed to concentrations of circulating neurotoxicants/PB as well as to any blood borne secondary influences (endocrine, immune) these agents might induce. As a result, vascular nociceptors could be the first.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2012
Accession Number
ADA590573

Entities

People

  • Brian Cooper

Organizations

  • University of Florida

Tags

DTIC Thesaurus Topics

  • Arteries
  • Blood
  • Cardiovascular System
  • Cells
  • Central Nervous System
  • Department Of Defense
  • Department Of Veterans Affairs
  • Diseases And Disorders
  • Medical Personnel
  • Membrane Potentials
  • Nerves
  • Nervous System
  • Pain
  • Persian Gulf Syndrome
  • Pharmacology
  • Pyridostigmine Bromide
  • Rodents

Fields of Study

  • Medicine

Readers

  • Gulf War Illness and Chronic Multisymptom Illness in Veterans.
  • Neurotrauma and Rehabilitation Medicine.
  • Trauma Surgery or Emergency Medicine.