Microvascular Channel Device to Study Aggressiveness in Prostate Cancer Metastasis
Abstract
To study circulating prostate cancer cell adhesion behavior and the contribution to distance metastasis of prostate cancer, we applied a dynamic flow-based E-selectin+SDF-1 coated microchannel system to isolate and characterize a subpopulation of adhering prostate cancer cells. We found a positive correlation of cells aggressiveness with their adhesion/rolling ability. By using this device we then sorted prostate cancer cell lines (PC3 and DU-145) into rolling and floating populations, and tested for their behavior in vitro and in vivo. Results from our in vitro studies found that rolling cancer cells are more aggressive in nature with higher growth rate and invasion and form bigger and more colonies in soft agar and 3D sphere formation assays. To test further in vivo, an orthotopic implantation mouse model is underway. In summary, this flow based device provides a platform for isolating the most dangerous aggressive form of cancer cells which can be used for human patients peripheral blood for isolating and characterizing and to form a process for customized cancer treatment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2013
- Accession Number
- ADA590794
Entities
People
- Sayeda Yasmin-karim
Organizations
- University of Rochester