The Function of Neuroendocrine Cells in Prostate Cancer
Abstract
The goal of the project is to determine the function of neuroendocrine (NE) cells in the initiation and progression of human prostate cancer 1) Use a pten null mouse prostate cancer model to determine if ablation of NE cells by selective expression of a toxin in these cells can delay or prevent tumor initiation and/or progression. 2) Use a human tissue recombination model to determine if depletion of NE cells from human epithelial cells can retard the initiation and progression of the recombinant tumor. 3). Demonstrating the origin and molecular basis of human small cell carcinoma Major findings: 1). We have successfully established a genotyping protocol to identify mice of the desired genotypes; 2). Expression of the toxin in tumor cells of TRAMP mice abolish tumor formation, demonstrating that the panned approach can ablate NE cells in mouse prostate tumors. 3). We have established a robust protocol to procure fresh human prostate tissue and isolate subpopulations of prostatic epithelial cells; 4). We have identified the appropriate combinations of cell surface markers for the isolation of NE cells; 5). Our initial study suggests that NE cells are not required for the propagation of established recombinant tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2012
- Accession Number
- ADA590965
Entities
People
- Jiaoti Huang
Organizations
- University of California, Los Angeles