Glyburide - Novel Prophylaxis and Effective Treatment for Blast-Traumatic Brain Injury
Abstract
The overall subject of this project is blast-traumatic brain injury (blast-TBI) and the role of the SUR1-regulated NCCa-ATP channel in blast-TBI. The specific objectives of this project include: (1) develop a standardized rat model of blast-TBI to study the direct transcranial effects of blast on the brain, independent of indirect transthoracic effects; (2) determine the role of the SUR1-regulated NCCa-ATP channel in blast-TBI; (3) in normal human volunteers, determine the safety of the SUR1 blocker, glyburide (glibenclamide), as it might be used as prophylaxis against blast-TBI. During the 4th year of this project we completed immunohistochemical analysis of the expression of the SUR1 and TRPM4 and neuroinflammatory markers in the rats subjected to blast-TBI (Objective 1b) induced by the Cranium Only Blast Injury Apparatus(COBIA), developed and calibrated at the beginning of the project (Obj. 1a). We completed evaluation of the prophylaxis treatment with SUR1 blocker, glyburide in the neurobehavioral outcome after blast-TBI (Obj. 1d, c). Results of the experiments show that administration of glyburide improves short term and long term neurobehavioral outcomes after Blast- TBI, and, compared to posttreatment, prophylaxis pretreatment with glyburide is more effective in preventing long term cognitive deficits.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA591278
Entities
People
- J. M. Simard
Organizations
- University of Baltimore