Innate Immunity Dysregulation in Myelodysplastic Syndromes
Abstract
We have proposed that an innate immune signaling axis formed by Toll-like receptor activation and maintained by the histone demethylase JMJD3 is deregulated in the bone marrow hematopoietic stem/ progenitor cells (HSPCs) of MDS. In this funding year, we have performed large scale expression and mutational analyses of key genes in this pathway in primary patient samples. We have achieved a systematic gene expression profiling about TLR1, 2, 6, JMJD3, IL8, and MYD88 in MDS. We have analyzed TLR2-F217S as a somatic mutation with biological gain-of-function property that occurs in 10% of 150 patients. Through clinical data analysis, we have defined associations of deregulation of TLR2-JMJD3 innate immunity genes with IPSS and survival of patients. At biological level, we have characterized the impact of TLR2 signaling in primary HSPCs, which indicated that abnormal activation of TLR2 inhibits erythroid differentiation. Finally, we have demonstrated that interference of TLR2-JMJD3 innate immunity signaling through inhibition of TLR2 and JMJD3 rescues the differentiation of erythroid lineage in patients with lower-risk diseases (low-risk and intermediate-1). In summary, we have achieved better understandings of the TLR2-JMJD3 innate immune pathway and its biology in MDS, including identification of potential bio-marks and novel therapeutic targets in this disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA591335
Entities
People
- Yue Wei
Organizations
- The University of Texas MD Anderson Cancer Center