Mutation of Breast Cancer Cell Genomic DNA by APOBEC3B

Abstract

Over the course of my tenure on this training grant, I have discovered that an endogenous enzyme, APOBEC3B, is upregulated in the majority of breast cancers. This holds true for both primary cancer tissues as well as cell lines. I have mechanistically characterized the enzyme s activity, sub-cellular localization, preferred sequence substrate and kinetics, and impact on the cancer cell genome. In addition, I have expanded my research to include all of the available cancer types represented in the publicly accessible portion of The Cancer Genome Atlas (TCGA). Using the massive data repository, I have found that the same mutational phenomenon that I have identified in breast cancer is also at work in 5 other types of cancer: head & neck cancer, cervical cancer, bladder cancer, squamous cell lung cancer and lung adenocarcinomas.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2013
Accession Number
ADA591365

Entities

People

  • Michael B Burns

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Bladder Cancer
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Colon Cancer
  • Effusion
  • Gene Expression
  • Genetic Variation
  • Genetics
  • Liquid Chromatography
  • Mass Spectrometry
  • Skin Cancer

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Molecular and genetic basis of cancer.