Dignital Biological Converter
Abstract
SGI has previously reported completion of the first three milestones. In this report we describe the successful demonstration of the fourth milestone fully automated generation of protein starting from a DNA sequence that codes for that protein. In our example, we produced green fluorescence protein (GFP). We entered a 1.5-kb gene sequence for GFP into SGI's Archetype that automatically generated a file containing the overlapping oligonucleotides necessary to produce the 1.5-kb gene fragment and initiated the start of the Digital-Biological Converter (DBC) run. Forty-eight overlapping ~64-base oligonucleotide oligonucleotides were synthesized, post-processed, and then pooled. The pooled oligonucleotides were then assembled and the resulting GFP gene fragment was amplified, error-corrected, and then re-amplified. This synthetic GFP gene product was then incubated in a cell-free extract and the synthetic GFP sequence was translated into protein. Multiple reactions were carried out in parallel and as much as 37 g/mL GFP protein was produced.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 03, 2014
- Accession Number
- ADA591563
Entities
People
- Heather Gouvis