The Snail-Induced Sulfonation Pathway in Breast Cancer Metastasis

Abstract

This project seeks to establish a key role for cellular sulfonation enzymes in the metastatic progression of breast cancer. To this end we have accomplished the following tasks in this second year of support: 1. Further characterization of Tetracycline inducible WT Snail and mutant Snail vectors and cell lines. 2. Demonstration that the PAPSS2 knockdown vectors do not work and/or are very leaky and must be further developed. 3. Demonstration MCF-10A cells undergo dramatic morphologic EMT in the presence of WT Snail expression, with the downregulation of the Cell adhesion molecule E-cadherin and the upregulation of the mesenchymal markers vimentin and fibronectin. 4. Characterization of antibodies to PAPSS1 and PAPSS2 enzymes and demonstration that they cross react with one another making their use in IHC very problematic. 5. Request for, and granting of an extension of this research project in order to solve the unexpected technical problems.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2013
Accession Number
ADA591987

Entities

People

  • Frank J. Rauscher Iii

Organizations

  • University of Pennsylvania

Tags

DTIC Thesaurus Topics

  • Acquisition
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Demonstrations
  • Department Of Defense
  • Gene Expression
  • Genes
  • Genetics
  • Mammary Glands
  • Metastasis
  • Molecules
  • Neoplasms
  • Proteins

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biochemistry
  • Oncology (Cancer Research).