Monoamine Oxidase A: A Novel Target for Progression and Metastasis of Prostate Cancer

Abstract

The purpose of this project is investigation of the critical role of the enzyme monoamine oxidase A (MAOA) in the progression and metastasis of prostate cancer. Throughout the Year 1 of this study we focused on study of the functional and mechanistic roles of MAOA in epithelial-to-mesenchymal transition (EMT), elevated production of reactive oxygen species (ROS), and its effect on hypoxia-inducible factor 1 (HIF1 ). We established and characterized stable wild-type and MAOA-knockdown human prostate cancer cells. Using our established cell lines we analyzed wild-type and MAOA-knockdown huhumanrostate cancer xenograft specimens. We also designed, developed and tested in vitro a novel pharmacological inhibitor of MAOA though chemical synthesis of the conjugate of MAO inhibitor clorgyline and near-infrared dye MHI-148. We found that inhibition of MAOA activity with this novel compound results in robust suppression of the prostate cancer cell growth and colony formation.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2013
Accession Number
ADA593309

Entities

People

  • Bogdan Z. Olenyuk
  • Jean C. Shih

Organizations

  • University of Southern California

Tags

DTIC Thesaurus Topics

  • Cancer
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Diseases And Disorders
  • Enzyme Inhibitors
  • Health Services
  • Inhibition
  • Inhibitors
  • Neoplasms
  • Oxidative Stress
  • Prostate Cancer
  • Therapy
  • Three Dimensional
  • Tissues

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Oncology (Cancer Research).