Ectopic Epithelial Deaminase in IBD
Abstract
This project is designed to dissect out the primary event that initiates the alteration of epithelial cell homeostasis in inflammatory bowel disease (IBD). Our hypothesis is that activation-induced cytidine deaminase (AID, a DNA-modifying enzyme), which is ectopically expressed in epithelial cells only under intestinal inflammatory condition, is primarily responsible for the initiation of epithelial homeostatic alteration through epigenetic modification. During the past budget period, we have successfully developed and expanded two key mouse strains that are necessary for testing our hypothesis. One mouse strain is a fate-mapping double reporter mouse system that allows us to closely examine epithelial cells with prior AID expression versus those without it. By utilizing this mouse system, we have picked up a signaling transducers and activators of transcription 3 (STAT3) as a candidate molecule that is targeted by AID. Another mouse strain is AID-deficient recombination activation gene (RAG) 1 double knockout mice that allow us to determine the functional role of AID in the pathogenesis of IBD.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA593311
Entities
People
- Atsushi Mizoguchi
Organizations
- Massachusetts General Hospital