Improving Blood Monitoring of Enzymes as Biomarkers of Risk from Anticholinergic Pesticides and Chemical Warfare Agents
Abstract
Blood biomarkers are an important way to monitor exposure to anticholinergic pesticides and chemical warfare agents. The widely used colorimetric Ellman assay is based on the hydrolysis of acetylthiocholine; CHPPM uses an equally reliable but slower delta pH method to monitor approximately 15,000 DOD personnel each year. Two different approaches were used to calculate conversion factors for expressing delta pH AChE in terms of Ellman assay units. First, paired samples were assayed by the delta pH method at CHPPM and by the Ellman method at UC Davis. Second, DFP-treated whole blood was used to mimic OP exposure. RBC AChE activity was measured using the Ellman and delta pH assays at UCD. The normal range of AChE activities from the CHPPM delta pH assay was converted to Ellman units, generating benchmarks for clinical determinations in the absence of baseline data. Conversion factors between Test Mate kit cholinesterase measurements and the delta pH and Ellman methods were also determined.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2007
- Accession Number
- ADA593418
Entities
People
- Barry W. Wilson
- John D. Henderson
Organizations
- University of California