Microbial Synthesis of 1,2,4-Butanetriol
Abstract
Efforts were made to improve the microbial synthesis of D-1,2,4-butanetriol from D-xylose previously constructed (Scheme 1). Benzoylformate decarboxylase variants from Pseudomonas putida (ATCC 12633) with 2-fold increase in kcat/Km values were generated using directed evolution. Pseudomonas fragi D-xylonate dehydratase was purified and sequenced. E. coli yjhG and yagF encoding D-xylonate dehydratases, and yjhH and yagE genes encoding 3-deoxy-D-glycero-pentulosonate aldolases were identified, respectively. Site-specific knockouts of the aldolases prevented the cleavage of intermediate 3-deoxy-D-glycero-pentulosonic acid to form pyruvic acid and glycolaldehyde. D-Xylose dehydrogenase from Caulobacter crescentus CB15 was identified and expressed in E. coli that converts D-xylose into D-xylonic acid.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2006
- Accession Number
- ADA593484
Entities
People
- John W. Frost
Organizations
- Michigan State University