Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up
Abstract
Use of resin-based extraction in a closed loop configuration with fermentor-controlled cultivation of phloroglucinol-synthesizing microbes presents numerous complications. Accordingly, microbial synthesis of phloroglucinol has been examined in the absence of resin-based extraction at both 2 L and 20 L scale. Both the relatively short catalytic lifetime of phlDencoded phloroglucinol synthase and the toxicity of phloroglucinol towards E. coli are additional challenges encountered during fermentor-controlled synthesis of phloroglucinol. Strategies for improving PhlD specific activity and extending the catalytic lifetime of PhlD in Escherichia coli were therefore examined. Activated sludge was explored as a source of microbes that are resistant to the toxicity of phloroglucinol.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2008
- Accession Number
- ADA593488
Entities
People
- John W. Frost