Exploring Neurofibromin Function in a Yeast Model of NF1
Abstract
We hypothesized that the NF1 protein Neurofibromin has functions in addition to its Ras-GAP activity and proposed to develop a yeast NF1 model to define new functions as well as novel regulators of known functions. In this 3 year project, we categorized genetic hits that we recovered from yeast screens. We divided hits into Ras-dependent and Ras-independent categories. We have identified proteins that physically interact with IRA1 and IRA2 (the yeast homologs of human Neurofibromin). We have also focused on a novel genetic interaction between IRA1 and IRA2 and yeast genes involved in peroxisome formation and function in yeast. These studies will illuminate the cellular pathways that Ira1 and Ira2 regulate and how neurofibromin deficiency contributes to disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2011
- Accession Number
- ADA593678
Entities
People
- Aaron Gitler
Organizations
- University of Pennsylvania