Quantitative Analysis of Cancer Cell Migration in Gradients Of EGF, HGF, and SDF-alpha Using a Microfluidic Chemotaxis Device

Abstract

We continued to develop microfluidic chambers to study breast cancer cell chemotaxis as it relates to metastasis. As part of this effort, we developed 3D culture devices that can generate concentration gradients of soluble factors in 3D gels, allowing cancer cell migration to be investigated in physiologically relevant conditions. We first developed devices capable of generating linear and non-linear gradients in 3D collagen gels. We then implemented these devices and developed a co-culture model to study the interaction between breast cancer cells and macrophages, each cultured in a distinct extracellular matrix that better simulates tissue composition. We studied the migration of these different cell types in these 3D devices. These studies suggested an intriguing synergy between tumor cells and immune cells, and pave the way for complex studies of cancer cell behavior in in vitro models that can better mimic tumor and tissue microenvironments.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2005
Accession Number
ADA593767

Entities

People

  • Noo L. Jeon

Organizations

  • University of California, Irvine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Processes
  • Blood
  • Breast Cancer
  • Cell Movement
  • Cells
  • Chemistry
  • Culture Techniques
  • Electronic Mail
  • Engineering
  • Fabrication
  • Geometry
  • Growth Factors
  • Stem Cells
  • Surface Properties
  • Surface Tension
  • Three Dimensional
  • Two Dimensional

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Immunology and Pathology
  • Nanocomposite Materials Science