Modulating Wnt Signaling Pathway to Enhance Allograft Integration in Orthopedic Trauma Treatment

Abstract

The research project was designed to test a novel approach of modulating Wnt signaling pathway in the bone tissue repair by using monoclonal antibodies against sclerostin (Sost) and DKK-1 (donated by Amgen Inc., Thousand Oaks, CA under MTA). Since the previous annual report, the project has progressed at a rapid pace. We resolved all initial technical difficulties and successfully completed all the surgical procedures, harvested samples at prescribe time points and evaluated new bone formation at the allograft site using CT scans and partially completed mechanical testing. Data presented in report reveals statistically that use of anti-Sost or anti-Dkk-1 antibodies enhances new bone formation around the allograft over all time points. Anti-Dkk-1 antibody treatment also seems to be superior to anti-Sost treatment. Mechanical testing show increasing strength over time. We are in the process of finalizing the mechanical testing and histology. Data so far supports our hypothesis.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2013
Accession Number
ADA594157

Entities

People

  • Amarjit S. Virdi

Organizations

  • Rush University

Tags

DTIC Thesaurus Topics

  • Allografts
  • Antibodies
  • Biomedical Research
  • Bone Regeneration
  • Data Analysis
  • Growth (Physiology)
  • Growth Factors
  • Osteogenesis
  • Peptide Growth Factors
  • Prostheses And Implants
  • Statistical Analysis
  • Surgery
  • X-Ray Computed Tomography

Readers

  • Immunology and Pathology
  • Systems Analysis and Design
  • Trauma Surgery or Emergency Medicine.