Enhancing the Breadth and Efficacy of Therapeutic Vaccines for Breast Cancer
Abstract
Over the last 12 months of this award, we have focused on the optimization of various systems for the identification of tumor-reactive T cells. Our team has tested and optimized protocols for the isolation of several immune cell types, the generation of antigen-presenting cells, and the culturing of T cells. We have also optimized protocols and systems to screen T cells for reactivity to cancer cell line antigens and the cloning of T cells. We have began screening patient T cells for reactivity to known antigens and to breast cancer cell line antigens, which will lead downstream to antigen discovery. We have also worked out conditions for direct T cell cloning based on TCR sequence analysis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594708
Entities
People
- Peter P Lee