Enhancing the Breadth and Efficacy of Therapeutic Vaccines for Breast Cancer
Abstract
The focus of the Spellman/Gray work group over the past year has been upon the generation of materials, tools, and data for the purpose of aiding and supporting the research and findings of the entire multi-team collaboration endeavoring to identify antigenic targets for breast cancer-infiltrating T cells. During this time, MHC-I-bound epitopes for several of our breast cancer cell lines were ranked and evaluated to determine cell surface-presenting, MHC I-loaded epitopes from genes with preferential or altered expression in breast cancer cell lines. An analytical pipeline was also developed to perform in silico epitope prediction using RNAseq data as input; the data from which contributes to the understanding of expressed tumor-associated protein-encoding transcripts. Additional RNAseq analysis was carried out to determine HLA-A2 typing of our in-house breast cancer cell lines. Finally, a small molecule drug compound plates have been generated and are ready to determine enrolled patients T cell IC50 for each compound.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594709
Entities
People
- Dmitri Rozanov
- Kami Chiotti
- Paul Spellman
Organizations
- Oregon Health & Science University