Role of Diet Modulation and AMPK in Ovarian Cancer Progression and Outcome
Abstract
Ovarian cancer is the leading cause of death from gynecologic malignancies in the United States. Given the ambiguity concerning the etiology, difficulties in early detection, absence of any markers, low survival rates and the overall obscurity related with ovarian cancer, more research is needed to identify factors and approaches that could improve ovarian cancer disease initiation, progression and disease free survival. One of these factors which have been largely unexplored is the influence of diet and the metabolic state of the patients. Unfortunately, the role of dietary factors in ovarian cancer prognosis is largely unknown. Particularly, no attention has been given to the metabolic state of a cancer cell and how this state can be modulated by calorie restriction. To determine the effect of diet on ovarian cancer, C57B6 mice were subjected to three types of diet: regular diet (RD), high-energy diet (HED) and calorie-restricted diet (CRD).. Post 30 days of diet, 5x106 ID8 mouse ovarian cancer cells were injected in the intra-peritoneal cavity and mice were sacrificed after 60 days, followed by tumor burden evaluation and physiological parameters. A set of mice were treated with metformin Compared to RD and CRD, HED fed mice showed the most extensive tumor nodule formation and the highest tumor score (diaphragm, peritoneum, bowel, liver, kidney, spleen) with higher levels of insulin and leptin in both ascites and serum compared to RD and CRD.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594736
Entities
People
- Ramandeep Rattan
Organizations
- Henry Ford Health