Dissecting an Estrogen Receptor-Regulated Enhancer Code in Breast Cancer
Abstract
The functional importance of gene enhancers in regulated gene expression is well established and recent evidence indicates that, in addition to widespread transcription of long non-coding RNA (ncRNA) transcripts in mammalian cells, bidirectional ncRNAs, referred to as eRNAs, are present on enhancer, However, it has remained unclear whether these eRNAs are functional, or merely a reflection of enhancer activation and may be a cause of upregulation of cancer genes. We found that breast cancer causing,17 -estradiol (E2)-bound estrogen receptor (ER ) on enhancers causes a global upregulation of eRNA transcription in enhancers adjacent to E2 upregulated coding genes in MCF-7 (Breast cancer cells). These induced eRNAs are required for the observed induction of target coding genes, causing the increased strength of specific enhancer:promoter looping, Our data indicate that eRNAs induced by liganded ER are required for effective recruitment of critical machinery required for estrogen regulated coding gene activation events and suggest that eRNAs are likely to exert important functions inmany regulated programs of gene expression in Breast cancer progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594737
Entities
People
- Dimple Notani
- Michael G. Rosenfeld
- Richard Schwab
Organizations
- University of California, San Diego