Enhancing the Breadth and Efficacy of Therapeutic Vaccines for Breast Cancer
Abstract
During the current funding period, we have made clear progress at the University of Colorado site and with the other teams at Oregon Health and Science University and City of Hope. The major objective of this project is to develop novel strategies to enhance the protective effects of anti-tumor T cells in vivo in breast cancer (bc) patients based on the hypothesis that partially protective anti-tumor T cells exist within in most bc patients. This year we focused on which T cells should be used for epitope identification in bc patients. We started to develop an assay in which we sequence the alpha and beta chain of single T cells in one PCR reaction. We will use this protocol from T cells that are positive in an IFN- + CD107a+ capture assay. This year we determined that culturing T cells in vitro results in significant skewing of the T cell repertoire. Thus, the sequence of the TCR of T cell clones expanded in culture must be compared with those isolated ex vivo. Finally we determined that T cells frequently present in the tumor and lymph node, but not frequently in the blood, should be analyzed. We are almost positioned to validate antigens and develop antigenic peptides recognized by T cells from bc patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594744
Entities
People
- Daniel J. Munson
- Jill E Slansky
- John W. Kappler
- Tullia C. Bruno
Organizations
- University of Colorado Health