Understanding Tumor Dormancy as a Means of Secondary Prevention
Abstract
The purpose of this collaborative project is to establish a molecular definition of the dormant state of a cancer cell. In doing this we hope to understand how this dormancy is broken, ultimately leading to recurrence in a patient that was stably in remission. Once our understanding of this is more complete it is hoped that we can devise strategies for secondary prevention. This funding year we have successfully optimized the two-photon microscopy for the identification of single, lone tumor cells within mice organs. We have devised new methodology to extract the single cells from a tissue section and have established how to use the current technology to extract RNA and make high quality transcriptome libraries from low input material. We anticipate additional advances in the quality of the transcriptome libraries from a single cell, fixed in PFA, as new technology is anticipated to be released before the end of the year. We have also made progress on strategies for identifying dormant cells in human biopsies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594797
Entities
People
- Gregory Hannon
Organizations
- Cold Spring Harbor Laboratory