Molecular Markers for Prostate Cancer Risk Stratification from Multiple Ultrasound-Guided Biopsies
Abstract
This goal of this project is to design and test a novel method for isolating cells from transrectal ultrasound-guided prostate cancer biopsy cores as they are extracted in the clinic and to do so in a manner that permits molecular characterization down to the single cell level, while maintaining the integrity of tissue cores for standard histopathological evaluation. We hypothesize that adding molecular markers, including recurrent patterns of genomic DNA rearrangement, mRNA and miRNA levels, and DNA point mutations, to current standard practice will significantly enhance risk stratification to appropriately guide treatment decisions. This is a pilot project to establish the feasibility and initial validation of a methodology that could serve as a bona fide clinical trial assay in future studies, while at the same time expanding our basic knowledge regarding the initiation, progression, and metastasis of the disease by charting the genetic relationships among individual cancer cells and the clonal cancer cell populations that develop into tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA594957
Entities
People
- James Hicks
Organizations
- Cold Spring Harbor Laboratory