Rapid Parallel Screening for Strain Optimization
Abstract
Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals, sequencing and annotation of isolated microbes was completed previously. Construction and optimization of reporter systems for testing candidate transcription factors was completed previously. Similarly, we have optimized the screening process so that empirical verification of transcription factor candidates is less labor intensive than originally envisioned. During this period we have finalized the identification of biodegradation gene clusters likely to be controlled by the desired transcription factors. Nearly 40 high quality candidates have been identified. DNA sequences representing transcription factors and their corresponding operators will now be assembled directly into the reporter system. Upon identification of a suitable and well-behaved transcription factor, the biosynthetic pathway for the corresponding biochemical will be installed and we will begin work on overproducing the molecule.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 18, 2014
- Accession Number
- ADA595268
Entities
People
- Chuck Merryman
- Tony Yee
Organizations
- J. Craig Venter Institute