Imaging Prostate Cancer Microenvironment by Collagen Hybridization
Abstract
We continue to derivatize and test the collagen mimetic peptides (CMPs) for imaging. Our proposed milestones in year two include: (1) Dual radio- and fluorescent labeling of CMPs retaining high-affinity (Kd 10-8 M) and specificity for intact and digested collagen (type I) films; (2) Validation of dual-labeled CMPs that display high affinity and specificity for stromal collagens in frozen PCa xenografts; and, (3) Measurement of pharmacokinetics and in vivo imaging of dual-labeled CMPs in mouse subcutaneous PC-3 xenograft models. Efforts have been focused on achieving a biologically stable CMP endowed with both gamma-emitting and fluorescent labels (milestone 1). To that end, we have employed several strategies utilizing the previously biologically validated, high affinity basic CMP peptide core, which may contain chelators, covalently bound radioiodine moieties and fluorescent dyes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA596668
Entities
People
- Martin G. Pomper
Organizations
- Johns Hopkins University