Efficacy and Safety of Frozen Blood for Transfusion in Trauma Patients
Abstract
Standard liquid red blood cells (SLRBCs) are approved for use up to 42 days. During this period, a known storage lesion occurs resulting in increased complications and death. We have previously shown that transfusion of cryopreserved deglycerolized RBCs (CDRBCs), which can be stored for 10 years, results in increased tissue oxygenation compared to SLRBCs. This study was performed to compare the biochemical properties and effects of CDRBCs to SLRBCs. Our hypothesis is that CDRBCs would have a similar profile with respect to the storage lesion compared to SLRBCs. Fifty-seven stable trauma patients who required one or two units of blood were randomized to receive SLRBCs or CDRBCs. Laboratory assays were performed on the donated blood and the patients prior to transfusion and on the patients 12 hours after transfusion. The median age of blood was 14 days in the SLRBC group and 21 months in the CDRBC group (p<0.01). Hematocrit increased similarly in both groups and remained similar throughout hospitalization. 2,3 diphosphoglycerate (2,3 DPG) was increased in patients who received CDRBCs and D-dimer levels increased in patients who received SLRBCs (p<0.05). Pro- and anti-inflammatory cytokines did not differ between groups after transfusion. Alpha-2 macroglobin, haptoglobin, C-reactive protein, and serum amyloid P were all higher in donated SLRBCs compared to CDRBCs (p<0.01). In conclusion, CDRBCs have superior characteristics with respect to the storage lesion compared to SLRBCs. Increases in 2,3 DPG are consistent with our prior findings of increased oxygenation in patients who receive CDRBCs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2012
- Accession Number
- ADA597687
Entities
People
- David A. Hampton
- Dinh Le
- Jennifer M. Watters
- Jerome A. Differding
- Laszlo Kiraly
- Loic J. Fabricant
- Martin A. Schreiber
- Samantha J. Underwood
Organizations
- Oregon Health & Science University