Molecular Profiles for Lung Cancer Pathogenesis and Detection in U.S. Veterans
Abstract
During our third year of research, we have successfully generated high quality mRNA sequencing and mRNA array data. Linear modeling followed by gene set enrichment analysis reveal connections in cancer gene expression throughout the airway. Specifically, we found that cancer genes in the distal airway are enriched in genes up in cancer in the mainstem bronchus. Similarly, cancer genes enriched in the bronchus and main carina show moderate enrichment in the nose. Our studies reveals spatially connected gene expression patterns in the airway of patients with lung cancer and demonstrated that the adjacent airway field of cancerization is comprised of markers that can identify lung cancer among smokers as well as gradient and localized site-dependent expression patterns that recapitulate NSCLC profiles. Our findings on the adjacent field of cancerization provide additional insights into the biology of NSCLC and the development of molecular tools for the detection of the malignancy. We have collected epithelial samples throughout the respiratory from smokers with and without lung cancer using common SOPs across all 4 participating institutions. We also used a unique approach to profile cell populations from the normal airway, premalignant lesions and tumors and were able to validate these genes. We have established proteomics methods required to validate our candidates in bronchial specimens during year 4 of the award.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA598341
Entities
People
- Pierre P. Massion
Organizations
- Vanderbilt University Medical Center