Three-Dimensional Ovarian and Oviductal Culture to Enhance Transgenic Animal Studies of Cancer and Prevention

Abstract

Our results confirm that single alterations in pathways associated with high-grade serous cancer are not sufficient to drive soft agar colony formation as an index of transformation, but that specific combinations such as KRAS/PTEN and mutant p53/PTEN, which represent some of the most common pathways activated in human high grade serous cancer, are able to promote early events in tumor formation. Mutation of p53 in the OSE or in the TEC is not sufficient to drive tumorogenesis; however, mutation of p53 combined with PTEN deletion enhances migration and soft agar colony formation. Similarly, PTEN silencing combined with KRAS mutation can enhance oviductal migration and enhance growth in soft agar. These data help support mounting evidence that oviductal epithelium can be a source of serous cancer and provide excellent models for future studies that are aimed at uncovering signaling events downstream of mutant p53, PTEN, KRAS, Rb, BRCA, and Akt.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2013
Accession Number
ADA598580

Entities

People

  • Joanna E. Burdette
  • Sharon L. Eddie
  • Suzanne M. Quartuccio

Organizations

  • University of Illinois at Chicago

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Cancer
  • Carcinoma
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Department Of Defense
  • Epithelial Cells
  • Epithelium
  • Genetic Engineering
  • Genetically Modified Organisms
  • Migration
  • Mutations
  • Neoplasms
  • Ovarian Cancer
  • Proteins
  • Three Dimensional

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.