Heparanase Mechanisms in Melanoma Brain Metastasis
Abstract
Objective of this application was to define the regulation of heparanase (HPSE) leading to a modulation of brain-metastatic melanoma (BMM). This goal was to be achieved by employing: a) novel HPSE inhibitors that either we have discovered, e.g., miR-1258, a microRNA targeting human HPSE and suppressing its expression, or have been recently developed, e.g., SST0001, a small molecule, membrane-permeable, nonanticoagulant glycol - split heparin and potent inhibitor of HPSE activity; b) a human BMM model recapitulating the stages of melanoma progression to the BMM phenotype; c) pINDUCER as novel inducible shRNA/cDNA expression lentiviral system which enables tracking of viral transduction and the uniform control of heparanase in cells and xenografts, thus minimizing experimental variations due to tumor heterogeneity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2013
- Accession Number
- ADA598581
Entities
People
- Dario Marchetti
Organizations
- Baylor College of Medicine