Synthetic Lethal Gene for PTEN as a Therapeutic Target

Abstract

PTEN is the gene which is down-regulated in the majority of prostate cancer, and PTEN-Akt pathway is considered to play a critical role in radiation resistance. The overall goal of this project is to identify a specific gene that is essential for this important pathway in the hope that targeting such gene will sensitize PTEN-negative tumor stem cells to be radiation-sensitive. To accomplish this goal, we will isolate cancer stem cells from patients and prepare PTEN-deficient cells. We will then screen genes that play critical roles in the PTEN pathway using a technique called shRNA library screening, with or without radiation treatment of these cells. When we identify a gene, we will then test the effect of such gene in an animal model to test our hypothesis. We believe that identifying such genes will lead to development of a novel therapeutic approach to radiation-resistant prostate cancer.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2013
Accession Number
ADA598763

Entities

People

  • Kounosuke Watabe

Organizations

  • University of Mississippi Medical Center

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Biological Sciences
  • Biomedical Research
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Department Of Defense
  • Diseases And Disorders
  • Information Operations
  • Materials
  • Medical Personnel
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Radiation
  • Resistance
  • Stem Cells

Readers

  • Nuclear and Radiation Engineering.
  • Oncology and Biomarker-Based Cancer Detection.
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology