Functional Proteomics to Identify Moderators of CD8+ T-Cell Function in Melanoma
Abstract
In the current funding period we have performed several rounds of phage-screens to select clones that differentially bind to either tumor infiltrating cytotoxic (CD8+) lymphocytes, activated CD8+ lymphocytes from the spleen, or un-activated na ve CD8+ T cells. We have developed a high-throughput flow cytometric approach that allows us to screen the specificity of several phage clones for each of these CD8+ populations. We have identified phage that selectively bind to na ve CD8+ T cells and those that bind to both tumor-infiltrating and effector CD8+ T cells, serving as a proof of principle for the strategy. We are making modifications to 1) the phage libraries that are screened and 2) the methods by which phage clones are selected for screening in order to increase the repertoire of phage that bind to TIL selectively.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2013
- Accession Number
- ADA599199
Entities
People
- Kimberly A. Kelly
- Timothy N. Bullock
Organizations
- University of Virginia