Regulation of Mammary Stem Cell Quiescence via Post-Translational Modification of DeltaNp63alpha

Abstract

This document is the annual summary report for the training grant awarded to Andrew DeCastro entitled Regulation of Mammary Stem Cell Quiescence via Post-Translational Modification of NP63alpha. Here, we report our findings under Task 3, as we have already completed our work on Tasks 1 and 2 in past years. Task 3 aims to determine the contribution of NP63alpha and NP63alpha-phosphorylation to therapeutic resistance in breast cancer stem cells. Based on or previous work, in which we show that TGF- mediates ALK5 dependent phosphorylation of NP63alpha, we also observed noticeable changes in cell phenotype indicative of EMT, a process utilized by cancer cells to mediate invasiveness, metastasis, chemoresistance and recurrence. As a result, we sought to determine the contribution of NP63alpha and its phosphorylation to the process of EMT and demonstrated that NP63alpha not only opposes TGF-beta induced EMT, but was sufficient to reverse a post-EMT breast cancer cell line back towards a more epithelial phenotype (MET). The data presented here identifies a role for NP63alpha as a potent oppose to TGF-beta mediated EMT.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2014
Accession Number
ADA599224

Entities

People

  • Andrew J. Decastro
  • James Direnzo
  • Pratima Cherukuri

Organizations

  • Dartmouth College

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Biological Sciences
  • Biology
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Genetics
  • Mammary Glands
  • Neoplasms
  • Proteins
  • Resistance
  • Stem Cells
  • United States

Readers

  • Breast cancer cell signaling and growth regulation.
  • Oncology (Cancer Research).
  • Technical Research and Report Writing.

Technology Areas

  • Biotechnology