Hetero-oligonucleotide Nanoscale Tiles Capable of Two-Dimensional Lattice Formation as Testbeds for a Rapid, Affordable Purification Methodology

Abstract

New nanoscale hetero-oligonucleotide tiles are assembled from DNA, RNA and morpholino oligos and purified using size exclusion filtration. Homo-oligonucleotide tiles assembled from RP-cartridge processed DNA oligos are purified by nondenaturing gel electro- phoresis. These tiles' purity and homogeneity are demonstrated by gel electrophoresis and their incorporation into two-dimensional arrays visualized by AFM. This purification methodology increases throughput and decreases costs for researchers who wish to screen multiple tiles for utilization in structural or analytical studies.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2013
Accession Number
ADA599245

Entities

People

  • Philip S. Lukeman

Organizations

  • St. John's University

Tags

Communities of Interest

  • Air Platforms
  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Abstracts
  • Assembly
  • Chemical Analysis
  • Chemistry
  • Crystal Lattices
  • Dna Microarrays
  • Dna Nanotechnology
  • Electronic Mail
  • Electrophoresis
  • Gel Electrophoresis
  • Homogeneity
  • Information Operations
  • Military Research
  • Molecular Weight
  • Nucleic Acids
  • Stoichiometry
  • Two Dimensional

Fields of Study

  • Biology

Readers

  • Aerospace Research.
  • Molecular Genetics
  • Molecular and Cellular Biochemistry