Role of Grainyhead in Kidney Cancer
Abstract
The cell of origin in clear cell renal cell carcinoma (ccRCC) is ambiguous. Previously, we had found through microarray data mining , that Grainyhead-like-2 (GRHL2) was expressed at much lower levels in ccRCC compared with normal kidney. In light of the relatively high overall expression of GRHL2 in the kidney, compared with other organs, we interpreted this result to mean that GRHL2 was down-regulated during oncogenic transformation. Our recent data, however, indicate that the much more likely interpretation is that ccRCC arises from GRHL2-negative cells of origin. These data include: a. low or undetectable expression levels of GRHL2 in either normal proximal or distal tubule-derived cell lines; b. low immunohistochemical signal for GRHL2 in normal proximal or distal tubules of human kidney samples; c. very high signal for GRHL2 in collecting duct and ureter. These results are highly significant because they show that ccRCC arises uniquely from GRHL2-negative but not GRHL2-positive ductal epithelial cells, informing a novel understanding of the biology of ccRCC.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA599266
Entities
People
- Steven Frisch
Organizations
- West Virginia University