Neuroprotection and Anti-Epileptogenesis with a Mitochondria-Targeted Antioxidant
Abstract
The goals of the project were to assess the neuroprotective and antiepiletogenic properties of a mitochondrial-targeted antioxidant, SS-31 using the pilocarpine (Pilo) model of status epilepticus (SE), the kindling seizure model and the tetanus toxin (Tx) model. Progress focused on Aim#1 due to limited access to SS-31. For Aim #2 the kindling model was established in the laboratory in preparation for drug testing. For Aim#1, adult male Sprague-Dawley rats (260-405g) were pretreated with SS-31 (3 or 10mg/kg, sc; n=14) or saline (n=10) 45min before induction of SE with Pilo (365mg/kg, sc). The latency to SE onset was measured for both groups. The brains were fixed 1-3 days after SE, sectioned through the hippocampus and stained for: Nissl, Fluoro-jade C (FJ), NeuN and heat shock protein (HSP). Three rats from each group died during SE. There was no significant difference in the latency to SE between the two groups. There was also no difference in Nissl, FJ, or NeuN staining between the groups. However one day after SE there was an increase in HSP staining in the granule cells of the dentate gyrus. These results suggest that SS-31 is not neuroprotective in the Pilo model but may be effective against less severe insults to the brain.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2013
- Accession Number
- ADA599267
Entities
People
- Jeffrey H. Goodman
Organizations
- Research Foundation For Mental Hygiene