Search and Neutralize Factors (CSPGs) that Induce Decline in Transmission to Motoneurons from Spared Fibers after Chronic Spinal Cord Injury
Abstract
Accomplishments during reporting Year 3. (1) We have conducted single-cell electrophysiological examination of transmission after chronic contusion SCI and obtained results demonstrating rewiring of corticospinal projections to individual interneurons and motoneurons. (2) The recombinant single chain (scFv) antibody has been created (patent pending), transferred to PENN vector core, where the new vector, i.e. AAV10 encoding the anti-NG2 scFv has been constructed. (3) Determined the optimal AAV vector serotype for NG2-Ab delivery: we examined expression pattern of six AAV-gfp serotypes (AV1,2,5,9,10,11) in damaged spinal cord; AAV10-gfp induced best transduction of spinal cord tissue following contusion SCI. (4) Examined AAV10-mediated delivery of NG2-Ab combined with neurotrophin NT-3 following SCI in rats and found significant recovery of function in treated vs control groups. Thus all specific aims are accomplished. Moreover, our study proved AAV10-NG2-Ab construct as novel effective and clinically relevant treatment to facilitate recovery after SCI. We apply for 6-moths no-cost extension to conduct immunochemistry analyses of spinal cord tissue from this completed experiment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA599392
Entities
People
- Victor Arvanian
Organizations
- Research Corporation