Maintenance of Paraoxonase 2 Activity as a Strategy to Attenuate P. Aeruginosa Virulence

Abstract

The P.aeruginosa signaling and virulence molecule 3OC12 mediates inactivation of the lactonase paraoxonase 2 (PON2) and induces many immunomodulatory effects in host cells. Because PON2 rapidly inactivates 3OC12, we hypothesized that preventing PON2 inactivation by 3OC12 could be a viable therapeutic strategy to limit P.aeruginosa quorum signaling and thereby attenuate virulence. We demonstrated in both human and mouse primary cell types that PON2 is sensitive to 3OC12-mediated inactivation at concentrations of 3OC12 expected to be present near P.aeruginosa colonies during infection. We also discovered that 3OC12 is rapidly hydrolyzed intracellularly by PON2 to 3OC12-acid, which becomes trapped and accumulates within the cells. 3OC12 caused a rapid cytosolic pH decrease, calcium release and phosphorylation of stress signaling kinases. All of these effects were dependent upon PON2 activity. A potent PON2 inhibitor was also identified and treatment of cells with the inhibitor prevented 3OC12-mediated inactivation of PON2. The findings suggest the intracellular acidification is that proximal event that mediates both PON2 inactivation and many immunomodulatory effects of 3OC12. Thus, protecting cells from 3OC12-mediated acidification could be an important therapeutic strategy to attenuate P.aeruginosa quorum signaling and virulence in infected individuals.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2013
Accession Number
ADA599512

Entities

People

  • John F. Teiber

Organizations

  • University of Texas at Dallas

Tags

DTIC Thesaurus Topics

  • Blood
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Confocal Microscopy
  • Culture Techniques
  • Cultured Cells
  • Endothelial Cells
  • Enzyme Inhibitors
  • Enzymes
  • Epithelial Cells
  • Infection
  • Inhibitors
  • Macrophages
  • Molecules
  • Organic Chemistry

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Microbial Pathology
  • Neurotoxicology