Biomarkers to Assess Possible Biological Effects on Reproductive Potential, Immune Function, and Energetic Fitness of Bottlenose Dolphins Exposed to Sounds Consistent with Naval Sonars
Abstract
The overarching goal of this research is to utilize novel biomarkers to examine whether significant sub-lethal responses to sonar-type sounds occur in bottlenose dolphins exposed to such sounds. The collaborators will use immune function markers, acute phase proteins, fertility potential assays, and targeted and non-targeted metabolomics to investigate samples collected from trained dolphins before exposure to the sonar-type sounds, immediately after exposure, and one week post-exposure. The tools we used included a range of biomarkers of effects, many of which have been developed and calibrated for human medical uses, but which work well in other mammals. The key is to design collaborative teams that acquire appropriate samples for analysis, conduct R&D to ensure that available assays can measure useful parameters, design experiments in which animals are humanely exposed to stressors and in which an extensive suite of biological effects is monitored, conduct chemical assays that rigorously adhere to QA/QC requirements, and interpret the results of assays with regard to what they do or do not do in terms of biologically significant effects on populations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 2011
- Accession Number
- ADA599825
Entities
People
- Dana L. Wetzel
Organizations
- Mote Marine Laboratory