Corneal Protection for Burn Patients
Abstract
The overall goal of this research is to preserve vision of patients recovering from severe facial burns by providing an improved method to reduce development of corneal defects, inflammation, infection and opacification. To improve and understand the properties of the degradation-resistant crosslinked amniotic membranes for treating cornea of burn patients, to evaluate a sutureless method for attaching membranes to cornea and construct a membrane-encapsulated contact lens. Identified three crosslinking methods that produced amnion with maximum protection against enzymatic degradation while remaining flexible enough to conform to cornea shape. Determined that protein crosslinking greatly reduces availability of beneficial factors in native amnion. Selected two crosslinking methods for in vivo evaluation. Demonstrated that crosslinked, degradationresistant amniotic membrane can be securely sealed to the corneal surface using a sutureless, light-activated technique. Constructed an amnion-encapsulated hydrogel lens to maintain hydration on the cornea surface.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA600481
Entities
People
- Irene E. Kochevar
Organizations
- Massachusetts General Hospital