A Novel Mechanism for the Pathogenesis of Nonmelanoma Skin Cancer Resulting from Early Exposure to Ultraviolet Light
Abstract
We proposed that skin egress, entering the circulation, and traveling throughout the body may be a characteristic of skin stem cells in response to ultraviolet light. We have made four significant findings in this regard: 1) murine epidermal keratinocytes migrate preferentially in vitro to whole living bone marrow with its conditioned medium, 2) keratinocytes migrate towards SDF1 alpha and HMGB1 baits over control attractants such as 3T3, medium without serum, and FBS, 3) CD184 (SDFR1 alpha) is expressed on approximately 27% of CD49f+/CD34+ hair follicle stem cells and suggesting that these cells may have the capacity to follow an SDF1alpha chemokine gradient, and 4) there is an increase in CD49f+/Keratin 14+ cells in blood and bone marrow following solar UV treatment as determined by FACS, immunofluorescence microscopy, and quantitative real-time PCR. The significance of these findings support our hypothesis that: 1) skin egress may be a characteristic of skin stem cells in response to ultraviolet light, and 2) bone marrow may be a long-lived reservoir of sunlight initiated stem cells that can repopulate the skin even years later upon damage caused by petrochemicals, skin wounding, or physical, chemical, or thermal damage to the skin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2013
- Accession Number
- ADA600686
Entities
People
- Rebecca Morris
Organizations
- University of Minnesota