Pro-lipogenic Action of Lysophosphatidic Acid in Ovarian Cancer

Abstract

The objective of the project was to determine the role of endogenous lysophosphatidic acid (LPA) in lipogenesis and metabolic abnormalities of ovarian cancer cells. We found that LPA upregulated de novo lipogenesis in ovarian cancer cells. We showed this effect of LPA is physiologically relevant by demonstrating the pro-lipogenic activity of endogenous levels of LPA. Suppression of the LPA-producing enzyme iPLA2beta strongly inhibited proliferation of ovarian cancer cells, an effect not reversed by the presence of LPA. Our results indicate the importance of fatty acid catabolism through beta oxidation in promotion of ovarian cancer cell growth and survival. Since we have shown that LPA-driven lipogenesis is required for proliferation of ovarian cancer cells, our results together establish a dual role for lipid metabolism (anabolism and catabolism) in maintenance of the malignant phenotype of ovarian cancer cells. The results from this pilot study have led to one publication in JBC and another manuscript in preparation. Built on the results of the study, we are also preparing an R01 grant application for submission to NIH/NCI in February of 2014.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2014
Accession Number
ADA600694

Entities

People

  • Xianjun Fang

Organizations

  • Virginia Commonwealth University

Tags

DTIC Thesaurus Topics

  • Anabolism
  • Breakpoint Temperature
  • Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Colon Cancer
  • Epithelial Cells
  • Fatty Acids
  • Gene Expression
  • Lipid Metabolism
  • Metabolism
  • Ovarian Cancer
  • Proteins

Fields of Study

  • Biology

Readers

  • Oncology (Cancer Research).
  • Prostate Cancer Biology.