Rapid Parallel Screening for Strain Optimization
Abstract
Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals, sequencing and annotation of isolated microbes was completed previously. Construction and optimization of reporter systems for testing candidate transcription factors was completed previously. Similarly, we have optimized the screening process so that empirical verification of transcription factor candidates is less labor intensive than originally envisioned. During this period we have finalized the identification of biodegradation gene clusters likely to be controlled by the desired transcription factors. Nearly 40 high quality candidates have been identified. Five DNA sequences representing transcription factors and their corresponding operators have been assembled and we will complete assembly of remaining candidates in batches of five. Although we need to verify genotypes, we will begin assaying responsiveness of the reporter to exogenously added inducers within two weeks. Upon identification of a suitable and well-behaved transcription factor, the biosynthetic pathway for the corresponding biochemical will be installed and we will begin work on overproducing the molecule.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 16, 2014
- Accession Number
- ADA600797
Entities
People
- Chuck Merryman
- Tony Yee
Organizations
- J. Craig Venter Institute