Discovery of a New Cellular Motion and Its Relevance to Breast Cancer and Involution

Abstract

Mechanisms establishing and controlling tissue polary are central both to normal breast function and breast cancer. Molecular studies have revealed key proteins that mediate and maintain cell- and tissue- polarity; however, the fundamental biophysical processes whi h a single cell must undergo multiple rounds of mitosis to assemble into polarized tissue-like structures and \ that are the fundamentals of loss of tissue architecture are hardly understood. We placed single non-malignent cells in 3-D laminin-rich (lrECM) cultures to observe what % of the cells could form 3-D acinar structures. Wsing high resolution real time imaging, we identified a morphogenic behavior where mammary epithelial eel undergo organized multiple rotations to establish an acinus (a sphere with hollow lumen). We named this Jlovement: .QOherent . ngular motion (CAMo). Here we are investigating the role of CAMo in normal development, aging and breast cancer progression.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2014
Accession Number
ADA600974

Entities

People

  • Mina Bissell

Organizations

  • University of California, Berkeley

Tags

DTIC Thesaurus Topics

  • Angular Motion
  • Breast Cancer
  • Cell Line
  • Cells
  • Confocal Microscopy
  • Culture Techniques
  • Department Of Defense
  • Epithelial Cells
  • High Resolution
  • Mammary Glands
  • Microscopy
  • Neoplasms
  • Polarity
  • Rotation
  • Three Dimensional
  • Tissues

Readers

  • Military Logistics and Supply Chain Management
  • Nanocomposite Materials Science
  • Neuroscience

Technology Areas

  • Biotechnology