Epigenetic Control of Tamoxifen-Resistant Breast Cancer

Abstract

The objective is to understand the role that epigenetics, specifically methylation, plays in antiestrogen resistant breast cancer. The goal of this study is to identify genes differentially methylated between acquired tamoxifen resistant cells (TMX2-28 and TMX2-11) and their parent strain (MCF-7) through the use of the Illumina HumanMethylation450 BeadChip. The most significant finding was that three genes found differentially methylated on the HumanMethylation450 BeadChip in both TMX2-11 and TMX2-28 as compared to MCF-7 had changes in expression when treated with 5-aza-2 deoxycitidine. Additionally, treatment with 5-aza-2 deoxycitidine affected the growth rate of the ER-negative Tamoxifen-resistant line, TMX2-28, but not MCF-7 or the ER-positive Tamoxifenresistant line, TMX2-11.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2014
Accession Number
ADA601260

Entities

People

  • Kristin P. Williams

Organizations

  • University of Massachusetts Amherst

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Apoptosis
  • Biological Sciences
  • Biology
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Chemistry
  • Culture Techniques
  • Drug Abuse
  • Epigenetics
  • Gene Expression
  • High Density
  • Human Factors Engineering
  • Medical Personnel
  • Neoplasms
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular and genetic basis of cancer.
  • Wave Propagation and Nonlinear Chaotic Dynamics.