SPANXB2 and Prostate Cancer Progression
Abstract
A critical problem in prostate cancer is our inability to reliably distinguish indolent from aggressive disease. Recent evidence has implicated a class of genes, termed Cancer Testis Antigens (CTA), in cancer progression. In preliminary studies, by crossing the CTA bank with a prostate cancer metastasis gene signature we attained from an orthotopical injection xenograft model, we postulated CTA SPANXB2 as a cancer metastasis related CTA. We observed that SPANXB2 is up-regulated in metastatic prostate cancer xenograft models and is induced upon exposure to stroma and stromal factors (i.e., TGF- ). We hypothesize that SPANX-B2 may be the key regulator of prostate cancer aggressive cell behavior and metastasis. In this report, for the first time, we illustrate that regulatory role of SPANXB2 in PC3 cells by using shRNA knockdown technique. Knockdown of SPANXB2 in PC3 cells significantly reduces the cell proliferation, migration, and invasion ability compared with the wild type PC3 cells. Additionally, co-culture of these knockdown cells with stromas partially rescues the phenotype. We also confirm that stromal cells promote cell aggressiveness in prostate caner cells and detect the TGF- 2 secretion is correlated to elevated SPANXB2 level in our epithelial-stromal model. The attained data provide solid basis for the second stage in vivo studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2013
- Accession Number
- ADA601287
Entities
People
- Hangwen Li
Organizations
- University of Michigan