The Role of PP2A Methylation in Susceptibility and Resistance to TBI and AD-Induced Neurodegeneration

Abstract

The focus of the current study is to test the effect of genetic manipulations that target the tau phosphatase, PP2A, on behavioral impairments resulting from shockwave exposure in a mouse model, and to compare those results with the effects of the same genetic manipulations on the sensitivity to AD-like impairments caused by acute beta-amyloid (A ) exposure. Our goal is to identify the molecular mechanisms that contribute to TBI or AD-related impairment so that this information can then be used to identify at-risk individuals and develop effective therapeutic approaches. The principal motivation behind this approach is the observation that aggregates of hyperphosphorylated tau are a common feature of multiple neurodegenerative conditions including Alzheimer s disease and traumatic brain injury-associated degeneration. We previously found that the two novel lines of transgenic mice will use in this study, altered sensitivity to A -induced electrophysiological and behavioral impairments, and our hypothesis is that they will exert similar effects on shocshockwave inducedairments. This effort has required a substantial investment in developing equipment and testing protocols for exposing mice to a range of shockwave exposure conditions that mimic militarily relevant exposures and then assessing the biochemical and behavioral consequences of those exposures. There is currently a pressing need for mouse models and methodologies that reproduce the key features of blast exposure observed in humans, and the results of our experiments are a significant contribution to that effort.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2013
Accession Number
ADA602077

Entities

People

  • Barclay Morrison
  • Christopher Hue
  • Edward K Vogel
  • Ottavio Arancio
  • Russell Nicholls

Organizations

  • Columbia University

Tags

DTIC Thesaurus Topics

  • Alzheimer Disease
  • Brain Injuries
  • Cell Membrane Structures
  • Chemical Synthesis
  • Chemistry
  • Cognitive Impairment
  • Confocal Microscopy
  • Diseases And Disorders
  • Genetic Engineering
  • Genetically Modified Organisms
  • Health Services
  • Medical Personnel
  • Metabolic Diseases
  • Neurodegeneration
  • Test Methods

Fields of Study

  • Biology

Readers

  • Auditory Neuroscience/Auditory Physiology.
  • Oncology (Cancer Research).
  • Traumatic Brain Injury (TBI) and Cognitive Aging in the Guam and Border Populations Affected by Alzheimer's Disease and Tau-Associated Dementias.

Technology Areas

  • Biotechnology