The Effect of Age on the Susceptibility and Severity of Demyelination
Abstract
The purpose of this investigation is to determine if aged mice are more susceptible to the induction of EAE an animal model for MS. EAE was induced in a cohort of mice (8 weeks old) which is the usual age for induction of chronic experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis. In addition cohorts of 2 aged C57Bl/6 mice ( 16 months,18 months and 20 months) were induced for the disease. A control mouse for each age was injected with all regents except for the myelin protein required to induce disease, myelin oligodendrocyte protein. The animals were then evaluated for the onset and severity of disease. None of the controls got sick up to 14 days after the initiation of EAE as expected. The onset of disease in the control cohort was at the expected time (9 days) after the initiation of EAE. However, on average the onset of EAE in all the aged animals occurred 2 to 6 days after the onset of EAE in the control group. The peak clinical disease score was 2.5 for the control group while in every aged cohort the clinical scores were 0.5 to 1.5 more severe. This preliminary data indicates that with increasing age EAE disease onset is later and more severe.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2013
- Accession Number
- ADA602684
Entities
People
- George H. Devries