Identification and Validation of Plasma Biomarkers in California Sea Lions

Abstract

This proposal focused on the discovery and validation of serum or plasma biomarkers for domoic acid toxicosis (DAT) in sea lions. Proteomic approaches were utilized to assess whether proteins could classify sea lions as DAT or non-DAT. Commercial cytokine arrays and MALDI-T of peptide profiling can be utilized as screening tools in the diagnosis of acute DAT, but will only detect about 25% of those sea lions with DAT. 2D gel electrophoresis studies demonstrated that Apolipoprotein E and eosinophil counts combined resulted in a perfect marker of DAT in training set samples. External validation supported this combination as a biomarker with a test sensitivity of 86% and specificity of 85%.

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Document Details

Document Type
Technical Report
Publication Date
Apr 24, 2014
Accession Number
ADA602905

Entities

People

  • Michael G. Janech

Organizations

  • Medical University of South Carolina

Tags

Communities of Interest

  • Autonomy

DTIC Thesaurus Topics

  • Blood
  • Blood Cells
  • Blood Proteins
  • Brain
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Gel Electrophoresis
  • Granulocytes
  • Machine Learning
  • Marine Mammals
  • Mass Spectrometry
  • Neural Networks
  • Polymer Chemistry
  • Proteins
  • Proteomics
  • Sea Lions

Readers

  • Molecular and Cellular Biochemistry
  • Molecular and genetic basis of cancer.
  • Psychometric Testing or Psychological Assessment.

Technology Areas

  • Biotechnology