Engineering Complex Microbial Phenotypes with Continuous Genetic Integration and Plasmid Based Multi-gene Library

Abstract

In this project we developed imaging systems based on the principles of compressive sensing. We developed advanced signal processing algorithms as well as a hardware testbed. The key idea in CS reconstruction is the realization that most signals encountered in practice are sparse in some domain and the theory of CS exploits such sparsity to dictate that far fewer sampling resources than traditional approaches are needed. Spectral image cubes are particularly well suited for sparse representation as images across different wavelengths exhibit strong correlation. We have thus developed a testbed to acquire and reconstruct natural images from a limited number of linear projection measurements at sub-Nyquist sampling rates. A key to the success of CS is the design of the measurement ensemble, which is based on the evaluation of the incoherence between the measurement ensemble and the sparsity basis. Due to the large scale nature of images, the generation of the measurement ensemble should be both computationally efficient and memory efficient. Based on these principles, we developed compressive cross-sectional imaging (confocal) systems, a single pixel compressive camera, and a multispectral imaging system. Advanced algorithms for the efficient reconstruction of the compressive measurements were developed.

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Document Details

Document Type
Technical Report
Publication Date
Oct 09, 2013
Accession Number
ADA603731

Entities

People

  • Eleftherios T Papoutsakis

Organizations

  • University of Delaware

Tags

DTIC Thesaurus Topics

  • Bioengineering
  • Biology
  • Biotechnology
  • Carrier Proteins
  • Cellular Structures
  • Engineering
  • Engineers
  • Escherichia Coli
  • Gene Expression
  • Genetic Structures
  • Genetics
  • Genome
  • Measurement
  • Medical Personnel
  • Proteins
  • Synthetic Biology
  • Virotherapy

Fields of Study

  • Physics

Readers

  • Adaptive Control and Estimation with Uncertainty in Dynamic Systems.
  • Image Processing and Computer Vision.

Technology Areas

  • Biotechnology