Origins of DNA Replication and Amplification in the Breast Cancer Genome

Abstract

The major goal of this IDEA Expansion Breast Cancer Research grant is to test whether a correlation exists between sites of DNA amplification and estrogen receptor (ER) binding in the breast cancer genome. Correlations would support our hypothesis that ER adjacent to replication origins may interact with the replication machinery to drive DNA amplification, a hallmark of many cancers. Our Specific Aims are: (1) Map replication origins in the MCF-7 breast cancer genome by genomic sequencing (2) Compare the replication origin maps between breast cancer (ER+, ER-) and normal breast cells (3) Correlate the origin map data with sites of DNA amplification and estrogen receptor binding (4) Pilot runs to map replication origins in ER+ human breast cancer tissue and sites of DNA amplification

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2011
Accession Number
ADA603896

Entities

People

  • Alexander Brodsky
  • Ben Raphael
  • Susan A. Gerbi

Organizations

  • Brown University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chromosomes
  • Computational Science
  • Computer Science
  • Data Sets
  • Department Of Defense
  • Estrogens
  • Genetics
  • Hormones
  • Human Genome
  • Medical Personnel
  • Neoplasms
  • Statistical Analysis
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics